Journal: Cell reports

Article Title: Differential Etv2 threshold requirement for endothelial and erythropoietic development

doi: 10.1016/j.celrep.2022.110881

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Single cell suspensions were then stained with a 1:100 dilution of Rat anti-mouse Ter119-PerCP or with a mix of rat anti-mouse CD45-PercP, rat anti-mouse CD11b-Pacific Blue, and rat anti-mouse CD93 at a 1:20 dilution (see ) for 45 min at 4°C with agitation and were then subjected to either flow cytometric analyses on a BD FACS Verse or BD FACS Aria III flow cytometer (BD Biosciences).

Techniques: RNA Sequencing Assay, ChIP-sequencing, Software

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: Immunoglobulin heavy chain gene-sequencing of single GAC+ B cells depicted as Circos plots (See Experimental Procedures). (A) GAC+ B cells (n=94) sorted from naïve adult C57BL/6 mice, (B) Adult C57BL/6 mice were immunized with GAS at d56 and GAC+ B cells were sorted 14 days post-immunization (d.p.i.) (n=74), (C) GAC+ B cells sorted from d56 resting adult C57BL/6 mice previously immunized as pups at d14 (n=87). IGH sequences are pooled from naïve (n=4), d56-immunized (n=4) or d14-immunized (n=4) mice from one representative experiment completed twice. Ribbon color coresponds IGHV identity (IGHV6 blue, IGHV7–3 red, all other IGHVs grey), IGHJ usage and IGHV mutations are depicted by inlayed heatmaps. (D) CDRH3 length distribution of pooled IGH gene sequences of identifiied IGHV families of GAC+ B cells. (E) CDRH3 consensus logos of of the mode CDRH3 length for pooled IGHV6–3 and IGHV7–3 IGH gene sequences. See also Figure S2. (F) Relative IGHV gene abundance in recall responses elicited from adult and d14 GAS-immunized mice following a prime-boost regimen. See also Figure S3. (G) Relative abundance of IGHV gene utilization of GAC+ B cells in d14, d21 and d28 neonates, adult (8–10wk) mice and aged adult (10–12mo) mice. (H) Relative IGHV gene abundance in AA4.1+ immature and AA4.1− mature GAC+ B cells isolated from d28 neonates. See also Figure S3.

Article Snippet: Rat monoclonal anti-Mouse CD93 (clone AA4.1), FITC , BD Biosciences , Cat: 559156.

Techniques: Sequencing, Isolation

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-Mouse CD93 (clone AA4.1), FITC , BD Biosciences , Cat: 559156.

Techniques: Recombinant, Sequencing, Software

Journal: eLife

Article Title: Plasmodium -specific atypical memory B cells are short-lived activated B cells

doi: 10.7554/eLife.39800

Figure Lengend Snippet:

Article Snippet: Antibody , Monoclonal Rat anti-Mouse CD93 (AA4.1) , eBioscience , 17–5892 , (dil 1/100).

Techniques: Produced, Activation Assay, Recombinant, Knock-In, Construct, Purification, Sequencing, Staining, Adjuvant, Software